The enzyme catalyzed reaction is continuous and fast, and offline sampling chromatography testing requires measures to quickly terminate the reaction, which affects the accuracy of the test, and the program is complex and the testing time is long.

The online Raman testing time is within a few seconds, which can continuously monitor the reaction process and quickly analyze process products, making it very suitable for monitoring biochemical synthesis reactions.

The following figure shows the concentration changes of substance A during the enzymatic reaction to produce substance B with the participation of the strain. The reaction was completed within the initial 2.7 minutes, and substance A was completely converted into substance B. At the 11th minute, the same dose of substance A was added again. The reaction was completed within 2 minutes, and the concentration of substance B doubled. This confirms the significant advantage of Raman online monitoring of biochemical reactions.